Last edited by Zulkilrajas
Tuesday, October 13, 2020 | History

5 edition of Antiviral RNAi found in the catalog.

Antiviral RNAi

Ronald P. van Rij

Antiviral RNAi

concepts, methods, and applications

by Ronald P. van Rij

  • 71 Want to read
  • 39 Currently reading

Published by Humana Press/Springer Science in New York .
Written in English

    Subjects:
  • RNA Interference,
  • RNA,
  • Gene Therapy,
  • Antiviral agents,
  • Small Interfering RNA,
  • Therapy,
  • Gene silencing,
  • Virus Diseases,
  • Antiviral Agents,
  • MicroRNAs

  • Edition Notes

    Includes bibliographical references and index.

    Statementedited by Ronald P. van Rij
    SeriesMethods in molecular biology -- v. 721, Springer protocols, Springer protocols, Methods in molecular biology (Clifton, N.J.) -- v. 721.
    Classifications
    LC ClassificationsRM411 .A5756 2011
    The Physical Object
    Paginationxi, 411 p. :
    Number of Pages411
    ID Numbers
    Open LibraryOL25166188M
    ISBN 101617790362
    ISBN 109781617790362
    LC Control Number2011923888
    OCLC/WorldCa690089348

    Small interfering RNA (siRNA)-mediated RNA interference (RNAi) pathways are critical for the detection and inhibition of RNA virus replication in insects. Recent work has also implicated RNAi pathways in the establishment of persistent virus infections and in the control of DNA virus replication. Accumulating evidence suggests that diverse double-stranded RNAs produced by RNA and DNA viruses Cited by: Authoritative and practical, Antiviral Resistance in Plants: Methods and Protocols seeks to aid scientists in the further study of this crucially important botanical trait. Keywords Antiviral Resistance Pathogen-mediated virus resistance Plant viral infections Plant-virus interactions RNA silencing Sub-viral RNAs Virus-induced gene silencing.

    Antiviral RNAi in Mammals While RNA silencing is crucial for antiviral immunity in plants and insects, there is no conclusive evidence supporting an antiviral function for RNAi in mammals. The detection of virus-derived siRNAs would provide direct evidence that viral sequences are cleaved by Dicer. The recent discovery of the positive-sense single-stranded RNA (ssRNA) Orsay virus (OV) as a natural pathogen of the nematode Caenorhabditis elegans has stimulated interest in exploring virus-nematode interactions. However, OV infection is restricted to a small number of intestinal cells, even in nematodes defective in their antiviral RNA interference (RNAi) response, and is neither lethal nor Cited by:

      Fighting Viruses with RNAi The long-debated issue of whether mammals can use RNA interference as an antiviral defense mechanism is finally put to rest. Y. Li et al., “RNA interference functions as an antiviral immunity mechanism in mammals,” Science, ,   The information on this page should be considered current for the influenza season for clinical practice regarding the use of influenza antiviral medications. Also see the current summary of recommendations available at Influenza Antiviral Medications: Summary for Clinicians and a list of related references at Antiviral References.


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Antiviral RNAi by Ronald P. van Rij Download PDF EPUB FB2

Antiviral RNAi: Concepts, Methods, and Applications provides a collection of protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as for the development and optimization of RNAi-based antiviral drugs.

As RNAi is a central regulatory mechanism in the cell, the methods in this volume can also be applied out of the context of a virus : Ronald P. van Rij. Authoritative and accessible, Antiviral RNAi: Concepts, Methods, and Applications serves as an ideal guide for both novice and experienced researchers alike striving to dissect the role of RNAi in the viral life cycle or to further boost the development of novel therapeutics and experimental tools based on RNAi.

Antiviral RNAi: Concepts, Methods, and Applications provides a collection of protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as for the development and optimization of RNAi-based antiviral drugs.

As RNAi is a central regulatory mechanism in the cell, the methods in this volume can also be applied out of the context of a virus infection. ANTIVIRAL RNAi: HOW TO SILENCE VIRUSES "Overall, the book is a clear success and represents an excellent and valuable treatise on current antiviral drug strategies.

It is a must for all medicinal chemists interested in the field of antiviral chemotherapy, and it would be a good acquisition for both institutional and personal libraries, alike."Pages: From the reviews:"This well-illustrated book details the necessary protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as the development and optimization of RNAi-based antiviral drugs.

The book is intended Antiviral RNAi book both Antiviral RNAi book and experienced researchers. ANTIVIRAL RNAi: HOW TO SILENCE VIRUSES The Discovery of RNA Interference Therapeutic Application of the RNAi Mechanism Mammalian Viruses and the RNAi Mechanism "Overall, the book is a clear success and represents an excellent and valuable treatise on current antiviral drug strategies.

It is a must for all medicinal chemists interested in. Initial studies investigating virus-C. elegans interactions used primary cell cultures and defined an antiviral role for the nematode RNA interference (RNAi) response [3, 4].

RNAi is a highly conserved mechanism of gene silencing that contributes to antiviral defense in insects [ 5 ], plants [ 6 ], and mammals [ 7, 8 ].Cited by:   The nematode Caenorhabditis elegans is an ideal model for the study of many aspects of cell biology, including the hot topic of RNA interference (RNAi Cited by:   Broad-spectrum antiviral inhibits novel coronavirus of NHC arises from increased mutation rate in viral genomic RNA.

In the case of MERS, treatment with 1. RNA interference (RNAi), a highly conserved gene silencing mechanism plays an important role in the regulation of gene expression.

This system was examined in a broad variety of species including plants, fungi, yeasts, nematodes, flies and mammals. In fact, RNAi serves as a safeguard for the preservation of genomic by: In arthropods, RNA interference (RNAi) is responsible for antiviral defense.

Here, we show that flies have a sophisticated systemic RNAi-based immunity mediated by macrophage-like haemocytes. Purchase Viral Polymerases - 1st Edition. Print Book & E-Book. ISBN  Antiviral RNAi in mammals.

(A) RNAi and the IFN response function at different times in mESCs.(B) Upon infection with an RNA virus (NoV) that lacks (NoVΔB2) or expresses a mutant (NoVmB2) viral RNAi suppressor, suckling mice mount an antiviral RNAi response that clears the lethality of wild-type (WT) NoV infections is likely due to viral suppression of antiviral RNAi Cited by: The RNA interference (RNAi) pathway is a potent antiviral defense mechanism in plants and invertebrates, in response to which viruses evolved suppressors of RNAi.

In mammals, the first line of defense is mediated by the type I interferon system (IFN); however, the degree to which RNAi contributes to antiviral defense is still not completely by: 4. An antiviral agent must act at one of five basic steps in the viral replication cycle in order to inhibit the virus: (1) attachment and penetration of the virus into the host cell, (2) uncoating of virus (e.g., removal of the protein surface and release of the viral DNA or RNA), (3) synthesis of new viral components by the host cell as directed.

Antiviral Resistance in Plants: Methods and Protocols (Methods in Molecular Biology): Medicine & Health Science Books @ RNA interference (RNAi) is also a major antiviral defense mechanism in arthropods. We have shown that Drosophila melanogaster strains defective in RNAi core components, Dicer2 and AGO-2, are highly hypersensitive to infection, resulting in a fold increase in virus production.

In turn, viruses have evolved mechanism to suppress RNAi. The antiviral effect of exogenous dsRNA inoculation required a functional RNAi machinery as Dicer 2 and Argonaute 2 null mutant flies (Dcr2-/-and Ago2-/ Cited by: Yet another antiviral technique inspired by genomics is a set of drugs based on ribozymes, which are enzymes that will cut apart viral RNA or DNA at selected sites.

In their natural course, ribozymes are used as part of the viral manufacturing sequence, but these synthetic ribozymes are designed to cut RNA and DNA at sites that will disable them. 6 RNA Interference-Mediated Intrinsic Antiviral Immunity in Plants interact with the P AZ and part of the PIWI-domain of AGO1, inhibiting the slic - ing activity of AGO1 (Zhang et al.

Broad Antiviral Function of Insect RNAi Recognition of dsRNA as a danger signal to induce an immune response is an effective strategy, since almost all viruses produce dsRNA during their replication cycle []. Therefore, antiviral RNAi is broadly active against a large number of RNA and DNA viruses in by: 4.

"Overall, the book is a clear success and represents an excellent and valuable treatise on current antiviral drug strategies. It is a must for all medicinal chemists interested in the field of antiviral chemotherapy, and it would be a good acquisition for both institutional and personal libraries, alike.".Approval of new antiviral drug brings hope and confusion North Texas hospitals welcome remdesivir — but worry about the drug’s availability and say more new treatments are needed.